A look inside: Cryo-EM Developments
Cryo-Electron Microscopy has emerged as one of the most powerful and insightful methods available to biomedical researchers. For this reason, Ohio State has made a significant investment in the purchase of two new cryo-EMs located at CEMAS. The 200 kV Glacios from Thermo Fisher Scientific is equipped with the Falcon III direct electron detector and will mainly be used for preliminary screening of cryo specimens. In addition to screening, the Glacios is a standalone single particle analysis data acquisition solution, and our instrument has collected high resolution maps below 3 Å. The Glacios is currently operational.
The Thermo Fisher Scientific Krios G3i is a 300 kV cryo-TEM equipped with a Gatan BioQuantum K3 imaging filter and a K3 direct electron detector. The Krios is also fitted with a Cs corrector and Volta phase plate, which further enhance the capabilities of the microscope. Briefly, the Krios arrived at CEMAS in late December 2018, and the final acceptance tests were completed at the end of March 2019. Installation of the camera and associated software was completed in late April. CEMAS staff are in the final stages of validating the microscope and are receiving additional training in microscope operation. At the end of July, staff members participated in an applications bootcamp, organized by Thermo Fisher Scientific and hosted by CEMAS, that covered the newest advances in cryo-EM. We are very excited to start collecting data on the Krios and look forward to reviewing project requests. Please reach out to CEMAS staff to discuss your cryo-EM needs.
