CRYO-ELECTRON MICROSCOPY (CRYO-EM)

CRYO-ELECTRON MICROSCOPY (CRYO-EM)
 

CEMAS is at the forefront in the convergence of physical and life sciences. Our multidisciplinary approach drives synergy and amplifies characterization capabilities to challenge "what is possible" in electron microscopy. Our comprehensive cryo-electron microscopy (cryo-EM) program advances this effort.

Located in a custom-designed facility, all cryo-EM instruments at CEMAS meet or exceed manufacturer performance specifications. CEMAS recently installed a Thermo Scientific Glacios Cryo-TEM with a Falcon III direct electron detector and a Ceta-D camera for MicroED analysis. In addition, CEMAS has a Thermo Scientific Krios G3i Cryo-TEM equipped with a Bioquantum imaging filter and a K3 direct electron detector, imaging phase plates, STEM/TEM tomography, and an image spherical aberration corrector. 

CEMAS can assist users in all aspects of the cryo-EM workflow, including initial negative stain analysis, cryogenic sample preparation, grid screening, high-resolution data collection, and preliminary data analysis.

To get started, apply to become a CEMAS user. View the Policies and Procedures for using the Cryo-Electron Microscopy facilities at CEMAS.

Cryo-EM Instruments at CEMAS

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glacios instrument
Thermo Scientific Glacios Cryo-TEM
In late 2018, CEMAS installed the Thermo Glacios cryoEM, which has a 200kv x-FEG source and can be configured for single particle analysis (SPA), tomography and other applications. Its capabilities include sample screening and data acquisition, and more.
/capabilities/cryo-em/thermo-scientific-krios-g3i-cryo-tem
Krios microscope
Thermo Scientific Krios G3i Cryo-TEM
In the spring of 2019, CEMAS installed the Thermo Scientific Krios G3i cryoEM, which has a 300 kV x-FEG source and can be configured for single particle analysis (SPA), tomography and other applications

Cryo-EM Techniques

Cryo-Electron Tomography
Single Particle Analysis
Micro Electron Diffraction
Cryo-EM News
Engineering cures for cancer
Cryo-EM capabilities on display in research aimed to combat sepsis
A look inside: Cryo-EM Developments
A better world through philanthropy and medical research

Ohio State Cryo-EM Interest Group

Researchers at Ohio State have organized an informal Cryo-EM Interest Group that meets 1-2 times per month. Meetings consist of a highlight talk lasting approximately 30 minutes, followed by a discussion about data analysis, sample preparation, and other cryo-EM focused topics. The group also welcomes outside speakers to talk about their research.

Are you interested in joining? Connect with CEMAS Cryo-EM Researcher Yoshie Narui at narui.2@osu.edu.